Programming of Poliovirus Inhibition of Deoxyribonucleic Acid Synthesis in HeLa Cells

Abstract

Ackermann , W. W. (The University of Michigan, Ann Arbor), and D. Wahl . Programming of poliovirus inhibition of deoxyribonucleic acid synthesis in HeLa cells. J. Bacteriol. 92: 1051–1054. 1966.—Deletion of arginine from a culture medium reduced the rate of deoxyribonucleic acid (DNA) synthesis in uninfected HeLa cells. The normal rate was promptly restored by addition of arginine. Deletion of arginine also prevented poliovirus from inhibiting DNA synthesis in HeLa cells. However, the inhibitory potential of the infection and the capacity of the host cell for stimulation with regard to DNA synthesis were both retained in arginine-depleted cells which were infected. Upon addition of arginine late in the infection, DNA synthesis was first stimulated and then inhibited.