Affiliation:
1. Department of Bacteriology, University of Wisconsin-Madison, Madison, Wisconsin 53706
Abstract
The carbon and electron flow pathways and the bacterial populations responsible for transformation of H
2
-CO
2
, formate, methanol, methylamine, acetate, glycine, ethanol, and lactate were examined in sediments collected from Knaack Lake, Wis. The sediments were 60% organic matter (pH 6.2) and did not display detectable sulfate-reducing activity, but they contained the following average concentration (in micromoles per liter of sediment) of metabolites and end products: sulfide, 10; methane, 1,540; CO
2
, 3,950; formate, 25; acetate, 157; ethanol, 174; and lactate, 138. Methane was produced predominately from acetate, and only 4% of the total CH
4
was derived from CO
2
. Methanogenesis was limited by low environmental temperature and sulfide levels and more importantly by low pH. Increasing in vitro pH to neutral values enhanced total methane production rates and the percentage of CO
2
transformed to methane but did not alter the amount of
14
CO
2
produced from [2-
14
C]acetate (∼24%). Analysis of both carbon transformation parameters with
14
C-labeled tracers and bacterial trophic group enumerations indicated that methanogenesis from acetate and both heterolactic- and acetic acid-producing fermentations were important to the anaerobic digestion process.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference41 articles.
1. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen;Abram J. W.;Arch. Microbiol.,1978
2. Intensity of bacterial methane formation in ooze deposits of certain lakes;Belyaev S. S.;Microbiology,1975
3. Enumeration of bacteria forming acetate from H2 and CO2 in anaerobic habitats;Braun M.;Arch. Microbiol.,1979
4. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a freshwater lake. I. Field observations. Antonie van Leeuwenhoek J;Cappenberg T. E.;Microbiol. Serol.,1974
5. Interrelations between sulfate-reducing and methane-producing bacteria in bottom deposits of a freshwater lake. III. Experiments with 14C-labeled substrates. Antonie van Leeuwenhoek J;Cappenberg T. E.;Microbiol. Serol.,1974
Cited by
136 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献