Affiliation:
1. Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164-7040
Abstract
ABSTRACT
Goats which have recovered from acute
Anaplasma ovis
infection remain seropositive, although infected erythrocytes cannot be detected by microscopic examination. Persistence of
A. ovis
17 to 21 months following experimental infection was demonstrated by PCR detection of the
msp-5
gene. Quantitative analysis of persistent rickettsemia over time showed that all levels were below the limit of microscopic detection and ranged from a low of 10
2
organisms/ml to peaks of 10
6
organisms/ml. Two patterns of persistent rickettsemia were observed: the first was characterized by cyclic fluctuations at 6- to 9-week intervals, similar to the pattern described for
A. marginale
-infected cattle, while in the second pattern, repetitive cycles did not occur and the rickettsemia levels were relatively constant. The
msp-2
and
msp-3
multigene families, which provide the genetic capacity for outer membrane protein antigenic variation during persistent
A. marginale
rickettsemia, were identified in the
A. ovis
genome by Southern blot analysis, and expression of an MSP-2 homologue was confirmed by using immunoblots.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
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