Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens-Reference-Cited by-同舟云学术

Preclinical Assessment of a Fully Automated Multiplex PCR Panel for Detection of Central Nervous System Pathogens

Published:2016-03 Issue:3 Volume:54 Page:785-787
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hanson K. E.¹²,Slechta E. S.³,Killpack J. A.⁴,Heyrend C.⁵,Lunt T.³,Daly J. A²⁵,Hemmert A. C.⁶,Blaschke A. J.⁴

Affiliation:

1. University of Utah Department of Medicine, Division of Infectious Diseases, School of Medicine, Salt Lake City, Utah, USA

2. University of Utah Department of Pathology, Clinical Microbiology, Salt Lake City, Utah, USA

3. ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah, USA

4. University of Utah Department of Pediatrics, Division of Pediatric Infectious Diseases, Salt Lake City, Utah, USA

5. Intermountain Primary Children's Hospital, Salt Lake City, Utah, USA

6. BioFire Diagnostics, LLC, Salt Lake City, Utah, USA

Abstract

ABSTRACT We evaluated a multiplexed PCR panel for the detection of 16 bacterial, viral, and fungal pathogens in cerebrospinal fluid. Panel results were compared to routine testing, and discrepancies were resolved by additional nucleic acid amplification tests or sequencing. Overall, the positive and negative agreements across methods were 92.9% and 91.9%, respectively.

Funder

Division of Intramural Research, National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.02850-15

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