Affiliation:
1. Department of Biochemistry and Cell Biology, Rice University, Houston, Texas 77005-1892
Abstract
ABSTRACT
A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in
Clostridium acetobutylicum
ATCC 824. The enzyme was overexpressed in
Escherichia coli
and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The
K
m
and
V
max
values for the substrate dihydroxyacetone phosphate were 0.53 mM and 1.56 mmol min
−1
μg
−1
, respectively. When
E. coli
glycerol dehydrogenase was coexpressed with methylglyoxal synthase in
E. coli
BL21(DE3), 3.9 mM 1,2-propanediol was produced.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
40 articles.
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