Affiliation:
1. Institut für Mikrobiologie der Universität Göttingen, D-3400 Göttingen, Federal Republic of Germany
Abstract
Methanosarcina barkeri
grew on methanol plus H
2
. Both substrates were consumed in equimolar amounts. Growth was strictly dependent on the presence of acetate, which was required for the biosynthesis of cellular constituents. Only about 0.4% of the methane produced originated from acetate. By using deuterated methanol, it was demonstrated that methanogenesis from this compound under H
2
did not occur via oxidation of methanol to CO
2
and subsequent reduction but by direct reduction with H
2
. Growth yields with methanol plus H
2
and with methanol alone were not significantly different: 2.8 g of cells per mol of methanol in mineral medium and 4.6 g of cells per mol of methanol in complex medium, respectively. Growth of
M. barkeri
on methanol plus H
2
depended strictly on the presence of sodium ions in the medium. In the presence of 50 mM K
+
the
K
s
for Na
+
was 5 mM.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
46 articles.
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