Affiliation:
1. Institut für Mikrobiologie der Gesellschaft für Strahlen- und Umweltforschung und der Universität, D-3400 Göttingen, Federal Republic of Germany
Abstract
3-Chlorobenzoate-grown cells of
Pseudomonas
sp. B13 readily cometabolized monofluorobenzoates. A catabolic pathway for the isomeric fluorobenzoates is proposed on the basis of key metabolites isolated. Only 4-fluorobenzoate was utilized and totally degraded after a short period of adaptation. The isoenzymes for total degradation of chlorocatechols, being found during growth with 3-chlorobenzoate or 4-chlorophenol, were not induced in the presence of fluorobenzoates. Correspondingly, only the ordinary enzymes of the benzoate pathway were detected in 4-fluorobenzoate-grown cells. Ring cleavage of 3-fluorocatechol was recognized as a critical step in 3-fluorobenzoate degradation. 2-Fluoro-
cis,cis
-muconic acid was identified as a dead-end metabolite from 2- and 3-fluorobenzoate catabolism. During 2-fluorobenzoate cometabolism, fluoride is eliminated by the initial dioxygenation.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference29 articles.
1. Stereochemistry of ,B-carboxy- and ,B-hydroxymethyl-muconic derivatives;Ainsworth A. T.;J. Chem. Soc. C, p.,1968
2. Blatt A. H. (ed.). 1943. 4-Aminoveratrole p. 44-46. In Organic syntheses vol. 2. John Wiley & Sons Inc. New York.
3. Nuclear magnetic resonance studies of 1,3-butadienes. I. The spectra of halogenated butadienes;Bothner-By A. A.;J. Am. Chem. Soc.,1965
4. Nuclear magnetic resonance studies of 1,3-butadienes. II. The relation of (H,H) coupling constants to conformation;Bothner-By A. A.;J. Am. Chem. Soc.,1965
5. Metabolism of monofluorobenzoate by Acinetobacter calcoaceticus N.C.I.B. 8250. Formation of monofluorocatechols;Clarke K. F.;Biochim. Biophys. Acta,1975
Cited by
103 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献