Biosynthesis of Phenylalanine from Phenylacetate by Chromatium and Rhodospirillum rubrum

Author:

Allison Milton J.1,Robinson Isadore M.1

Affiliation:

1. National Animal Disease Laboratory, Agricultural Research Service, Ames, Iowa

Abstract

Cultures of Chromatium strain D and Rhodospirillum rubrum incorporated 14 C from phenylacetate- 1 - 14 C during anaerobic growth. The radioactivity in the protein fraction of cells was mainly in phenylalanine. Phenylalanine from Chromatium cells grown in phenylacetate- 1 - 14 C was labeled at carbon 2. Incorporation of phenylacetate by Chromatium was decreased in the presence of exogenous phenylalanine, and de novo synthesis of phenylalanine from bicarbonate was less in medium containing either phenylalanine or phenylacetate. These organisms, and also certain anaerobic rumen bacteria, apparently carboxylate phenylacetate to synthesize the phenylalanine carbon skeleton. The mechanism of the carboxylation is unknown; however, it appears to be dependent upon anaerobic conditions, since R. rubrum did not synthesize phenylalanine from phenylacetate during aerobic growth in the dark.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference39 articles.

1. Phenylalanine biosynthesis from phenylacetic acid by anaerobic bacteria from the rumen;ALLISON M. J.;Biochem. Biophys. Res. Commun.,1965

2. ALLISON M. J. 1966. Biosynthesis of phenylalanine from phenylacetate by anaerobic bacteria. Proc. Intern. Congr. Microbiol. 9th Moscow U.S.S.R. p. 240.

3. Biosynthesis of branched-chain amino acids from branched-chain fatty acids by rumen bacteria;ALLISON M. J.;Arch. Biochem. Biophys.,1963

4. Metabolic function of branched-chain volatile fatty acids, growth factors for ruminococci. Il. Biosynthesis of higher branched-chain fatty acids and aldehydes;ALLISON M. J.;J. Bacteriol.,1962

5. The biosynthesis of alanine by Clostridium kluyveri;ANDREW I. G.;Biochim. Biophys. Acta,1965

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