Affiliation:
1. National Animal Disease Laboratory, Agricultural Research Service, Ames, Iowa
Abstract
Cultures of
Chromatium
strain D and
Rhodospirillum rubrum
incorporated
14
C from phenylacetate-
1
-
14
C
during anaerobic growth. The radioactivity in the protein fraction of cells was mainly in phenylalanine. Phenylalanine from
Chromatium
cells grown in phenylacetate-
1
-
14
C
was labeled at carbon 2. Incorporation of phenylacetate by
Chromatium
was decreased in the presence of exogenous phenylalanine, and de novo synthesis of phenylalanine from bicarbonate was less in medium containing either phenylalanine or phenylacetate. These organisms, and also certain anaerobic rumen bacteria, apparently carboxylate phenylacetate to synthesize the phenylalanine carbon skeleton. The mechanism of the carboxylation is unknown; however, it appears to be dependent upon anaerobic conditions, since
R. rubrum
did not synthesize phenylalanine from phenylacetate during aerobic growth in the dark.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
10 articles.
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