Author:
Garrett S,Taylor R K,Silhavy T J,Berman M L
Abstract
We isolated a series of delta ompR delta envZ mutants by inducing a strain carrying a lambda prophage in the closely linked gene malP and screening the bacterial survivors for loss of the major outer membrane porins OmpF and OmpC. Characterization of these deletion strains showed that both OmpR and EnvZ were necessary for transcription of ompF and ompC and that neither gene was essential for cell viability. Moreover, the deletion strains did not exhibit the pleiotropic membrane protein deficiency observed with certain envZ mutants. The method described should allow the simple isolation of deletions in any region of the chromosome.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference33 articles.
1. Linkage map of Escherichia coli K-12, edition 7;Bachmann B. J.;Microbiol. Rev.,1983
2. Outer membrane proteins of Escherichia coli. VI. Protein alteration in bacteriophage-resistant mutants;Bassford P. J.;J. Bacteriol.,1977
3. Beck E. G. Ludwig E. A. Averswald B. Reiss and H. Schaller. 1982. Nucleotide sequence and exact localization of the 844 NOTES
4. Transposition of R-factor genes to bacteriophage X;Berg D. E.;Proc. Natl. Acad. Sci. U.S.A.,1975
5. Positive selection for loss of tetracycline resistance;Bochner B. R.;J. Bacteriol.,1980
Cited by
61 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献