Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2-Reference-Cited by-同舟云学术

Evaluation of Nucleocapsid and Spike Protein-Based Enzyme-Linked Immunosorbent Assays for Detecting Antibodies against SARS-CoV-2

Published:2020-05-26 Issue:6 Volume:58 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Liu Wanbing¹,Liu Lei¹,Kou Guomei¹,Zheng Yaqiong¹,Ding Yinjuan¹,Ni Wenxu¹,Wang Qiongshu²,Tan Li²,Wu Wanlei¹,Tang Shi¹,Xiong Zhou¹,Zheng Shangen¹

Affiliation:

1. Department of Transfusion, General Hospital of Central Theater Command of the People’s Liberation Army, Wuhan, Hubei, China

2. Department of Disease Control and Prevention, General Hospital of Central Theater Command of the People’s Liberation Army, Wuhan, Hubei, China

Abstract

At present, PCR-based nucleic acid detection cannot meet the demands for coronavirus infectious disease (COVID-19) diagnosis. Two hundred fourteen confirmed COVID-19 patients who were hospitalized in the General Hospital of Central Theater Command of the People’s Liberation Army between 18 January and 26 February 2020 were recruited. Two enzyme-linked immunosorbent assay (ELISA) kits based on recombinant severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid protein (rN) and spike protein (rS) were used for detecting IgM and IgG antibodies, and their diagnostic feasibility was evaluated.

Funder

China Postdoctoral Science Foundation

National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00461-20

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