Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions-Reference-Cited by-同舟云学术

Genomic Characterization of Cyanophage vB_AphaS-CL131 Infecting Filamentous Diazotrophic Cyanobacterium Aphanizomenon flos-aquae Reveals Novel Insights into Virus-Bacterium Interactions

Published:2019-01 Issue:1 Volume:85 Page:
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Šulčius Sigitas¹²,Šimoliūnas Eugenijus³,Alzbutas Gediminas¹⁴⁵,Gasiūnas Giedrius⁶⁷,Jauniškis Vykintas⁶⁸,Kuznecova Jolita¹,Miettinen Sini⁹,Nilsson Emelie²,Meškys Rolandas³,Roine Elina¹⁰,Paškauskas Ričardas¹,Holmfeldt Karin²

Affiliation:

1. Laboratory of Algology and Microbial Ecology, Nature Research Centre, Vilnius, Lithuania

2. Center for Ecology and Evolution in Microbial Model Systems, Department of Biology and Environmental Science, Linnaeus University, Kalmar, Sweden

3. Department of Molecular Microbiology and Biotechnology, Institute of Biochemistry, Vilnius University, Vilnius, Lithuania

4. Thermo Fisher Scientific Baltics, Vilnius, Lithuania

5. Faculty of Mathematics and Informatics, Vilnius University, Vilnius, Lithuania

6. Department of Protein-DNA Interactions, Institute of Biotechnology, Vilnius University, Vilnius, Lithuania

7. CasZyme, Vilnius, Lithuania

8. Protein Structure and Function Programme, Structural Molecular Biology Group, Novo Nordisk Foundation Center for Protein Research, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark

9. Proteomics Unit, Institute of Biotechnology, Helsinki Institute of Life Science, University of Helsinki, Helsinki, Finland

10. Molecular and Integrative Biosciences Research Programme, University of Helsinki, Helsinki, Finland

Abstract

The genomic characterization of novel cyanophage vB_AphaS-CL131 and the analysis of its genomic features in the context of other viruses, metagenomic data, and host CRISPR-Cas systems contribute toward a better understanding of aquatic viral diversity and distribution in general and of brackish-water cyanophages infecting filamentous diazotrophic cyanobacteria in the Baltic Sea in particular. The results of this study revealed previously undescribed features of cyanophage genomes (e.g., self-excising intein-containing putative dCTP deaminase and putative cyanophage-encoded CRISPR-Cas and toxin-antitoxin systems) and can therefore be used to predict potential interactions between bloom-forming cyanobacteria and their cyanophages.

Funder

Lietuvos Mokslo Taryba

Svenska Forskningsrådet Formas

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.01311-18

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