Activation of Bacteriophage Mu mom Transcription by C Protein Does Not Require Specific Interaction with the Carboxyl-Terminal Region of the α or ς 70 Subunit of Escherichia coli RNA Polymerase-Reference-Cited by-同舟云学术

Activation of Bacteriophage Mu mom Transcription by C Protein Does Not Require Specific Interaction with the Carboxyl-Terminal Region of the α or ς 70 Subunit of Escherichia coli RNA Polymerase

Published:1998-06-15 Issue:12 Volume:180 Page:3257-3259
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Sun Weiyong¹,Hattman Stanley¹,Fujita Noboyuki²,Ishihama Akira²

Affiliation:

1. Department of Biology, University of Rochester, Rochester, New York 14627,1 and

2. Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan2

Abstract

ABSTRACT Late in its growth cycle, transcription of the phage Mu mom promoter (P mom ) is activated by the phage gene product, C, a site-specific DNA binding protein. In vitro transcription analyses showed that this activation does not require specific contacts between C and the carboxyl-terminal region of the α or ς 70 subunit of Escherichia coli RNA polymerase. Unexpectedly, these results are in contrast to those known for another Mu-encoded transcriptional activator, Mor, which has a high degree of sequence identity with C and appears to interact with the carboxyl termini of both α and ς 70 .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.180.12.3257-3259.1998

Reference40 articles.

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5. Role of bacteriophage Mu C protein in activation of the mom gene promoter

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3. Identification of the domains for DNA binding and transactivation function of C protein from bacteriophage Mu;Proteins: Structure, Function, and Genetics;2003-06-23

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