Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by Xba I Restriction and Resolved by Pulsed-Field Gel Electrophoresis-Reference-Cited by-同舟云学术

Reproducibility of Bordetella pertussis Genomic DNA Fragments Generated by Xba I Restriction and Resolved by Pulsed-Field Gel Electrophoresis

Published:2002-03 Issue:3 Volume:40 Page:811-816
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Hardwick Terri H.¹,Plikaytis Brian²,Cassiday Pamela K.¹,Cage Gary³,Peppler Mark S.⁴,Shea Deborah⁵,Boxrud David⁶,Sanden Gary N.¹

Affiliation:

1. Meningitis and Special Pathogens Branch

2. Biostatistics and Information Management Branch, Division of Bacterial and Mycotic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia

3. Department of Clinical Microbiology, Arizona Department of Health Services, Phoenix, Arizona

4. Canada Department of Medical Microbiology and Immunology, Edmonton, Alberta, Canada

5. State Laboratory Institute, Diagnostic Laboratories, Massachusetts State Public Health Laboratory, Jamaica Plain, Massachusetts

6. Microbiology Laboratory, Minnesota Department of Health, Minneapolis, Minnesota

Abstract

ABSTRACT The intra- and interlaboratory variabilities of the molecular size measurements of each DNA fragment contributing to three pulsed-field gel electrophoresis (PFGE) profiles were assessed, as were the reproducibilities of the entire PFGE profiles for three Bordetella pertussis strains. The major source of variability within a laboratory occurred between subcultures rather than within gels or between gels. Each PFGE profile was generated reproducibly and was objectively defined by the molecular sizes of its composite fragments. A strain or profile most suitable for use as an internal reference standard was identified.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.3.811-816.2002

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