Gram Staining: a Comparison of Two Automated Systems and Manual Staining

Author:

Froböse Neele J.1,Bjedov Sara1,Schuler Franziska1,Kahl Barbara C.1ORCID,Kampmeier Stefanie2,Schaumburg Frieder1ORCID

Affiliation:

1. Institute of Medical Microbiology, University Hospital Münster, Münster, Germany

2. Institute of Hygiene, University Hospital Münster, Münster, Germany

Abstract

Various Gram staining automated systems are available to accelerate and standardize the staining process, but a systematic comparison of different systems is largely lacking. The objective of this study was to evaluate two devices in comparison to manual Gram staining. Clinical samples ( n  = 500; University Hospital Münster, Germany; May to June 2020) were simultaneously Gram stained manually and with two automated Gram stainers (Previ Color Gram, bioMérieux, and ColorAX2, Axonlab).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference8 articles.

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2. Gram HCJ, Friedlaender C. 1884. Ueber die isolirte Färbung der Schizomyceten: in Schnitt-und Trockenpräparaten. Fortschritte Der Medicin, vol. 2, p 185–189. Theodor Fischer's medicinischer Buchhandlung, Berlin, Germany.

3. Targeting errors in microbiology: the case of the Gram stain

4. Clinical Utility of an Automated Instrument for Gram Staining Single Slides

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