Heat Shock Induction of Manganese Peroxidase Gene Transcription in Phanerochaete chrysosporium

Abstract

The expression of manganese peroxidase (MnP) in nitrogen-limited cultures of Phanerochaete chrysosporium is regulated by heat shock at the level of gene transcription. Nitrogen limitation and manganous ion [Mn(II)] previously have been shown to regulate mnp gene transcription. Northern (RNA) blot analysis demonstrates that 45°C heat shock results in the accumulation of mnp mRNA, even in cells grown in the absence of Mn. Heat shock induces mnp gene transcription in 4- or 5-day-old cells, and mnp mRNA is detectable after 15 min at 45°C. Maximum accumulation of mnp mRNA is observed 1 to 2 h after transfer of cultures to 45°C. Two hours after heat shock-induced cultures grown in the absence of Mn are transferred back to 37°C, mnp mRNA is no longer detectable. Higher levels of mnp mRNA are obtained with simultaneous induction by Mn and heat shock than by either treatment alone. Neither MnP enzyme activity nor protein is detectable in heat-shocked cultures grown in the absence of Mn. However, higher MnP activity is found in the extracellular medium of cultures induced by both heat shock and Mn than in the medium of cultures induced by Mn alone. These results suggest that the putative heat shock elements found in the promoter region of the mnp genes are physiologically functional and that Mn may be required for a posttranscriptional step of MnP production under heat shock conditions.