Affiliation:
1. Institut für Medizinische Mikrobiologie, Medizinische Hochschule Hannover, Germany.
Abstract
We report on the identification of mutations associated with streptomycin resistance in Mycobacterium tuberculosis. Two isolates (3656 and 3976) showed a wild-type ribosomal protein, S12, but exhibited a single point mutation at 16S rRNA position 491 (C-->T) or 512 (C-->T), respectively. Sequence analysis of a third isolate (2438) revealed a single base change at 16S rRNA position 904 (A-->G). This position is equivalent to invariant position 913 of the Escherichia coli 16S rRNA gene, an A-->G transition of which has been shown previously to impair streptomycin binding and streptomycin-induced misreading in vivo. Surprisingly, strain 2438 harbors an additional mutation in the ribosomal protein S12 (Lys-88-->Gln).
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference49 articles.
1. Mechanisms of antibiotic resistance in bacteria. Annu;Benveniste R.;Rev. Biochem.,1973
2. Blanc M. C. A. Adams and D. C. Wallace. 1981. Different
3. Analysis of streptomycin resistance of Escherichia coli mutants;Bonny C.;Biochem. Biophys. Acta,1991
4. The dominance of streptomycin sensitivity reexamined;Breckenridge L.;Proc. Natl. Acad. Sci. USA,1969
5. Genetic analysis of streptomycin resistance in Escherichia coli;Breckenridge L.;Genetics,1970
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