Affiliation:
1. Department of Microbiology, Scripps Clinic and Research Foundation, La Jolla, California 92037
Abstract
In examining the medium used in cultivation of
Mycoplasma
for deoxyribonucleic acid isolation, it was found that an aggregate was present which sedimented with the organisms and which was ethyl alcohol-precipitated during deoxyribonucleic acid purification. To eliminate the contaminating material, a method was devised to obtain only the dialyzable constituents of the medium. This report describes the preparation of a dialysate of soy peptone-yeast extract. The medium, obtained by immersion of the encased dehydrated ingredients in sodium chloride solution for 5.5 hr at approximately 80 C, has been employed as the basal medium for cultivation of a number of
Mycoplasma
species. Comparative growth curves of two saprophytic strains and two parasitic species indicated that multiplication in dialysate, with suitable supplement, followed the pattern typical of the common eubacteria. Thus, by elimination of the sediment which occurred in nondialyzed medium,
Mycoplasma
could be concentrated without concomitant accumulation of contaminating macromolecules.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
17 articles.
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