T-cell-mediated protection of mice against virulent Mycobacterium tuberculosis

Author:

Leveton C1,Barnass S1,Champion B1,Lucas S1,De Souza B1,Nicol M1,Banerjee D1,Rook G1

Affiliation:

1. Department of Medical Microbiology, University College, London, United Kingdom.

Abstract

We sought to protect CBA mice against tuberculosis using in vivo transfer of a T-cell line previously shown to be capable of I-A-restricted recognition of peritoneal macrophages infected in vitro with Mycobacterium tuberculosis. This line induces total bacteriostasis in vitro. In mice that received 500 rads of irradiation 48 h before infection, the T-cell line caused significant prolongation of life when given intravenously with a challenge dose of 5 x 10(6) organisms. Similar experiments with two other T-cell lines showed that these lines offered no protection. Bacterial load at the time of death was inversely related to the time of survival. Thus, death occurred at a lower bacterial load in adoptively protected mice, implying the contribution of an immunopathological component in these animals. The protective T-cell line, which was CD4+ CD8-, had no effect on the rate of growth of strain BCG in CBA nu/nu mice or M. tuberculosis in fully T-cell-deprived mice. This could indicate that CD8+ cells play a role in this system or that there is a need for the recruitment of interleukin 2-producing cells in the recipient. Experiments with monoclonal antibodies to selectively deplete T-cell subsets in normal CBA mice showed that depletion of CD4+ cells strikingly shortened survival, whereas depletion of CD8+ cells did not. However, CD8-depleted mice died with a lower bacterial load than those found in nondepleted controls, and the lesions in CD8-depleted mice were histopathologically distinct. These results suggest that the CD8+ cells either down-regulate bacteriostasis or cause immunopathology in this model and that it is the CD4+ cells that are the major protective subset in long-term protection experiments.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference14 articles.

1. The effect of Iymphokines on the ability of macrophages to protect mycobacteria from a bactericidal antibiotic;Altes C.;Tubercle,1985

2. Autoreactive T-cell lines specific for mouse thyroglobulin;Champion B. R.;Immunology,1985

3. Monoclonal antibodies for the prevention of graft-versus-host disease and marrow graft rejection. The depletion of T cell subsets in vitro and in vivo;Cobbold S.;Transplantation,1986

4. Therapy with monoclonal antibodies by elimination of T-cell subsets in vivo;Cobbold S. P.;Nature (London),1984

5. Cell-mediated immunity in mice vaccinated against malaria;Cottreli B. J.;Clin. Exp. Immunol.,1978

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