Pichia anomala DBVPG 3003 Secretes a Ubiquitin-Like Protein That Has Antimicrobial Activity

Author:

De Ingeniis Jessica12,Raffaelli Nadia1,Ciani Maurizio2,Mannazzu Ilaria23

Affiliation:

1. Istituto di Biotecnologie Biochimiche, Università Politecnica delle Marche, Via Ranieri, 60131 Ancona, Italy

2. Dipartimento SAIFET sez di Microbiologia Alimentare, Industriale e Ambientale, Università Politecnica delle Marche, Via Brecce Bianche, 60131 Ancona, Italy

3. Dipartimento di Scienze Ambientali Agrarie e Biotecnologie Agroalimentari, Università degli Studi di Sassari, Viale Italia 39, 07100 Sassari, Italy

Abstract

ABSTRACT The yeast strain Pichia anomala DBVPG 3003 secretes a killer toxin (Pikt) that has antifungal activity against Brettanomyces/Dekkera sp. yeasts. Pikt interacts with β-1,6-glucan, consistent with binding to the cell wall of sensitive targets. In contrast to that of toxin K1, secreted by Saccharomyces cerevisiae , Pikt killer activity is not mediated by an increase in membrane permeability. Purification of the toxin yielded a homogeneous protein of about 8 kDa, which showed a marked similarity to ubiquitin in terms of molecular mass and N-terminal sequences. Pikt is also specifically recognized by anti-bovine ubiquitin antibodies and, similar to ubiquitin-like peptides, is not absorbed by DEAE-cellulose. However, Pikt differs from ubiquitin in its sensitivity to proteolytic enzymes. Therefore, Pikt appears to be a novel ubiquitin-like peptide that has killer activity.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference56 articles.

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