A molecular analysis of meropenem–vaborbactam non-susceptible KPC-producing <i>Klebsiella pneumoniae</i>-Reference-Cited by-同舟云学术

A molecular analysis of meropenem–vaborbactam non-susceptible KPC-producing Klebsiella pneumoniae

Published:2024-08-20 Issue: Volume: Page:
ISSN:0066-4804
Container-title:Antimicrobial Agents and Chemotherapy
language:en
Short-container-title:Antimicrob Agents Chemother

Author:

Yasmin Mohamad¹^ORCID,Marshall Steven H.¹,Chen Liang²^ORCID,Rhoads Daniel D.³^ORCID,Jacobs Michael R.⁴^ORCID,Rojas Laura J.¹⁵⁶^ORCID,Perez Federico¹,Hujer Andrea M.¹⁷,Hujer Kristine M.¹⁷,van Duin David⁸^ORCID,Fowler Vance⁹,Chambers Henry F.¹⁰,Kreiswirth Barry N.²^ORCID,Bonomo Robert A.¹⁵⁶⁷¹¹^ORCID,

Affiliation:

1. Louis Stokes Cleveland Department of Veterans Affairs Medical Center, Cleveland, Ohio, USA

2. Center for Discovery and Innovation, Hackensack Meridian Health, Nutley, New Jersey, USA

3. Department of Pathology, Cleveland Clinic Lerner College of Medicine, Cleveland, Ohio, USA

4. Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

5. Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

6. Case Western Reserve University–Cleveland VAMC Center for Antimicrobial Resistance and Epidemiology (Case VA CARES), Cleveland, Ohio, USA

7. Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

8. Department of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA

9. Duke Clinical Research Institute, Duke University School of Medicine, Durham, North Carolina, USA

10. Department of Medicine, University of California San Francisco, San Francisco, California, USA

11. Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio, USA

Abstract

ABSTRACT We characterized the molecular determinants of meropenem–vaborbactam (MV) non-susceptibility among non-metallo-β-lactamase-producing KPC- Klebsiella pneumoniae (KPC- KP ). Whole-genome sequencing was performed to identify mutations associated with MV non-susceptibility. Isolates with elevated MV MICs were found to have mutations encoding truncated or altered OmpK36 porins and increased bla KPC copy numbers. KPC- KP isolates with decreased susceptibility to MV were detected among a collection of isolates predating the availability of MV.

Funder

HHS | NIH | National Institute of Allergy and Infectious Diseases

Publisher

American Society for Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/aac.00208-24

Reference9 articles.

1. Treatment for carbapenem-resistant Enterobacterales infections: recent advances and future directions

2. New Carbapenemase Inhibitors: Clearing the Way for the β-Lactams

3. Resistance to Novel β-Lactam–β-Lactamase Inhibitor Combinations

4. Microbiological, Clinical, and PK/PD Features of the New Anti-Gram-Negative Antibiotics: β-Lactam/β-Lactamase Inhibitors in Combination and Cefiderocol—An All-Inclusive Guide for Clinicians

5. Surveillance of Carbapenem-Resistant Klebsiella pneumoniae: Tracking Molecular Epidemiology and Outcomes through a Regional Network