Affiliation:
1. Department of Food Science, North Carolina State University, Raleigh, North Carolina 27607
Abstract
Lactobacilli in fecal material from humans, pigs, and chickens were enumerated on lactobacillus selective agar (LBS). In all samples, higher numbers of lactobacilli were detected when plates were incubated in a system flushed with CO
2
rather than in air. Much higher numbers of bacteria from human feces were detected when the LBS agar plates were incubated anaerobically in a hydrogen-carbon dioxide atmosphere (GasPak) than when incubated in CO
2
. The bacteria from human feces isolated on LBS agar incubated anaerobically were predominately bifidobacteria. Cultures from all three sources isolated on LBS agar incubated under CO
2
were lactobacilli, including
Lactobacillus acidophilus
. Differences were observed in biochemical characteristics of some of the
L. acidophilus
isolated from all three sources. Guanine plus cytosine base ratios of deoxyribonucleic acid isolated from
L. acidophilus
cultures from humans were lower, in most cases, than those from pigs and chickens.
Publisher
American Society for Microbiology
Subject
General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
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