Thermal Inactivation Characteristics of Bacterial Spores at Ultrahigh Temperatures

Author:

Busta F. F.1

Affiliation:

1. Department of Food Science, North Carolina State University, Raleigh, North Carolina 27607

Abstract

The thermal inactivation characteristics of Bacillus stearothermophilus (1518) spores and putrefactive anaerobe (PA) 3679 (NCA) spores suspended in skim milk were determined after treatment in pilot-plant ultrahigh-temperature (UHT) processing equipment. Temperature-survivor curves were constructed from survival data to emphasize the critical nature of temperature control in process evaluation. Time-survivor curves for PA 3679 spores were concave upward, and decimal reduction time (DRT) curves for these spores supported the observation of a protective response occurring at the longest exposure times. However, exposure time did not markedly affect the extremely high z D value obtained for PA 3679 spores. The substitution of Gelysate for Trypticase and Thiotone as the peptone in the sporulation medium increased the relative heat resistance of B. stearothermophilus spores, but lowered the z D value from 16 F to 12 F. The DRT curves in all cases were linear, but the z D values observed in this study differed considerably from those reported by other workers.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference20 articles.

1. Heat adaptation and ion exchange in Bacillus megaterium spores;ALDERTON G., P.;Science,1964

2. Effect of divalent cations in the sporulation medium on the thermal death rate of Bacillus coagulans var. thermoacidurans;AMAHA M.;J. Bacteriol.,1957

3. AMERICAN PUBLIC HEALTH ASSocIATION. 1960. Standard methods for the examination of dairy products 11th ed. American Public Health Association Inc. New York.

4. AMERICAN PUBLIC HEALTH AssoCIATIoN. 1965. Standard methods for the examination of water and wastewater including bottom sediments and sludges 12th ed. American Public Health Association Inc. New York.

5. Enumeration of Bacillus stearothermophilus by use of membrane filter techniques to eliminate inhibitors present in milk;BUSTA F. F.;Appl. Microbiol.,1965

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