Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans-Reference-Cited by-同舟云学术

Assessment of an In-House Enzyme-Linked Immunosorbent Assay and IgG Avidity Test Based on SAG1 and GRA7 Proteins for Discriminating between Acute and Chronic Toxoplasmosis in Humans

Published:2021-07-19 Issue:8 Volume:59 Page:
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Teimouri Aref¹,Abbaszadeh Afshar Mohammad Javad²³,Mohtasebi Sina²,Jafarpour Azami Sanaz²,Alimi Rasoul⁴,Keshavarz Hossein²⁵^ORCID

Affiliation:

1. Department of Parasitology and Mycology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran

2. Department of Medical Parasitology and Mycology, Tehran University of Medical Sciences, Tehran, Iran

3. Department of Medical Parasitology and Mycology, School of Medicine, Jiroft University of Medical Sciences, Jiroft, Iran

4. Healthy Ageing Research Center, Neyshabur University of Medical Sciences, Neyshabur, Iran

5. Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran

Abstract

To improve serodiagnostic methods for diagnosis of acute from chronic toxoplasmosis, an economical in-house enzyme-linked immunosorbent assay (ELISA) for measuring Toxoplasma -specific IgG, IgM, and IgG avidity has been developed and assessed based on use of various Toxoplasma gondii antigens, including SAG1, GRA7, and a combination of SAG1 and GRA7 (SAG1+GRA7), as well as Toxoplasma lysate antigens (TLAs). Performances of in-house IgM, IgG, and IgG avidity assays were compared to those of ELISA commercial kits and VIDAS Toxo IgG avidity.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.00416-21

Reference51 articles.

1. Dubey JP. 2010. Toxoplasmosis of animals and humans, 2nd ed. CRC Press LLC, Boca Raton, Florida

2. Toxoplasma gondii: from animals to humans

3. Toxoplasmosis

4. Epidemiology of and Diagnostic Strategies for Toxoplasmosis

5. Recombinant Antigens To Detect Toxoplasma gondii -Specific Immunoglobulin G and Immunoglobulin M in Human Sera by Enzyme Immunoassay

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