Affiliation:
1. Genetics Program, Michigan State University, East Lansing 48824-1101.
Abstract
The filamentous soil bacterium Streptomyces coelicolor is known to produce four antibiotics which are genetically and structurally distinct. An extensive search for antibiotic regulatory mutants led to the discovery of absB mutants, which are antibiotic deficient but sporulation proficient. Genetic analysis of the absB mutants has resulted in definition of the absB locus at 5 o'clock on the genetic map. Multiple cloned copies of the actII-ORF4 gene, an activator of synthesis of the antibiotic actinorhodin, restore actinorhodin biosynthetic capability to the absB mutants. These results are interpreted to mean that the failure of absB mutants to produce antibiotics results from decreased expression of the antibiotic genes. The absB gene is proposed to be involved in global regulation of antibiotic synthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference38 articles.
1. Mutations in a new Streptomyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation;Adamidis T.;J. Bacteriol.,1990
2. Transcriptional and translational features of a sporulation gene of Streptomyces griseus;Babcock M. J.;Gene,1990
3. Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in Streptomyces coelicolor A3(2);Bibb M. J.;J. Gen. Microbiol.,1981
4. Phage vectors that allow monitoring of transcription of secondary metabolism genes in Streptomyces;Bruton C.;Bio/Technology,1991
5. New loci required for Streptomyces coelicolor morphological and physiological differentiation;Champness W.;J. Bacteriol.,1988
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