Comparative evaluation of a modified zinc sulfate flotation technique

Author:

Bartlett M S,Harper K,Smith N,Verbanac P,Smith J W

Abstract

A modified zinc sulfate flotation technique using Formalinized fecal specimens (F-ZnSO4) was compared to the Formalin-ether (FE) concentration method for the laboratory diagnosis of intestinal parasites. Many laboratories have difficulty storing, using, and disposing of either and need a procedure for concentrating fecal specimens which does not require ether. Comparative data were obtained for the recovery of protozoan cysts and helminth eggs and larvae from feces preserved in Formalin less than and longer than 1 month. Whereas the FE method was found generally to be more efficient, F-ZnSO4 was apparently more effective for the recovery of some species of parasites. F-ZnSO4 was not satisfactory for recovery of schistosome eggs. We conclude that, except for schistosomes, F-ZnSO4 compares favorably to the FE method for detecting infections of clinical significance.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference7 articles.

1. Dobell C. and F. W. O'Connor. 1921. Intestinal protozoa of man. William Wood New York.

2. A critical study of clinical laboratory techniques for the diagnosis of protozoan cysts and helminth eggs in feces;Faust E. C.;Am. J. Trop. Med.,1938

3. Harper K. 1964. Routine laboratory procedures for intestinal parasitology. Procedure manual. Microbiology Division Bureau of Laboratories Indiana State Board of Health.

4. Melvin D. M. and M. M. Brooke. 1974. Laboratory procedures for the diagnosis of intestinal parasites. U.S. Department of Health Education and Welfare publication no. (CDC) 75-8282 Atlanta Ga.

5. A comparison of the zinc sulfate and the MGL (formalinether) technics;Ritchie L. S.;J. Parasitol.,1952

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