Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca 2+ Transients in Ciliated Tracheal Epithelial Cells-Reference-Cited by-同舟云学术

Functional BvgAS Virulence Control System in Bordetella bronchiseptica Is Necessary for Induction of Ca 2+ Transients in Ciliated Tracheal Epithelial Cells

Published:2003-12 Issue:12 Volume:71 Page:7208-7210
ISSN:0019-9567
Container-title:Infection and Immunity
language:en
Short-container-title:Infect Immun

Author:

Groathouse Nathan A.¹²,Heinzen Robert A.¹³,Boitano Scott¹⁴

Affiliation:

1. Department of Zoology and Physiology and Department of Molecular Biology, University of Wyoming, Laramie, Wyoming 82701

2. Mycobacteria Research Laboratories, Department of Microbiology, Colorado State University, Fort Collins, Colorado 80523-1682

3. Laboratory of Intracellular Parasites, Rocky Mountain Laboratories, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana 59840

4. Department of Physiology, Arizona Respiratory Center, University of Arizona, Tucson, Arizona 85724-5030

Abstract

ABSTRACT To study initial Bordetella bronchiseptica -tracheal epithelial cell interactions, we coincubated B. bronchiseptica with rabbit tracheal explant cultures and assayed bacterial adherence and host cell Ca 2+ signaling. Wild-type B. bronchiseptica (RB50) preferentially adhered to cilia and induced ciliated host cell Ca 2+ transients within 2 min of coincubation, whereas coincubation with an avirulent strain (RB57) resulted in limited binding and Ca 2+ signaling. The described cell system allows for assessment of initial B. bronchiseptica -host cell interactions that can contribute to pathogenicity or to host cell defense.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Link

https://journals.asm.org/doi/pdf/10.1128/IAI.71.12.7208-7210.2003

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