Cytotoxic Effects In Vitro of Highly Purified Streptolysin O on Mouse Macrophages Cultured in a Serum-Free Medium

Abstract

Fauve, Robert M. (Institut Pasteur, Garches (Hauts de Seine), France), Joseph E. Alouf, Albert Delaunay, and Marcel Raynaud . Cytotoxic effects in vitro of highly purified streptolysin O on mouse macrophages cultured in a serum-free medium. J. Bacteriol. 92: 1150–1153. 1966.—After the addition of highly purified streptolysin O to mouse macrophages cultured in medium 199 containing albumin, the following cellular alterations were observed. The disappearance of the stellate shape of the cells and the formation of bleb-like vesicles extruding from the cytoplasmic membrane occurred within a few minutes. Later, massive degranulation and formation of cytoplasmic vesicles were followed by cell death.