Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA-Reference-Cited by-同舟云学术

Nonradioactive PCR-enzyme-linked immunosorbent assay method for detection of human cytomegalovirus DNA

Published:1995-03 Issue:3 Volume:33 Page:725-728
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Allen R D¹,Pellett P E¹,Stewart J A¹,Koopmans M¹

Affiliation:

1. Viral Exanthems and Herpesvirus Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.

Abstract

We developed a rapid, sensitive, and specific PCR-based assay for human cytomegalovirus (HCMV). The assay includes primer and probe sequences derived from conserved HCMV nucleotide sequences and nonradioactive hybridization-confirmation. The assay detected between 10 and 100 viral genomes. All HCMV clinical isolates tested (39 of 39) gave positive reactions.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/jcm.33.3.725-728.1995

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