Affiliation:
1. Department of Molecular Genetics, Weizmann Institute of Science, Rehovot 76100, Israel
Abstract
ABSTRACT
Polarized growth in the budding yeast
Saccharomyces cerevisiae
depends upon the asymmetric localization and enrichment of polarity and secretion factors at the membrane prior to budding. We examined how these factors (i.e., Cdc42, Sec4, and Sro7) reach the bud site and found that their respective mRNAs localize to the tip of the incipient bud prior to nuclear division. Asymmetric mRNA localization depends upon factors that facilitate
ASH1
mRNA localization (e.g., the 3′ untranslated region, She proteins 1 to 5, Puf6, actin cytoskeleton, and a physical association with She2). mRNA placement precedes protein enrichment and subsequent bud emergence, implying that mRNA localization contributes to polarization. Correspondingly, mRNAs encoding proteins which are not asymmetrically distributed (i.e., Snc1, Mso1, Tub1, Pex3, and Oxa1) are not polarized. Finally, mutations which affect cortical endoplasmic reticulum (ER) entry and anchoring in the bud (
myo4
Δ,
sec3
Δ, and
srp101
) also affect asymmetric mRNA localization. Bud-localized mRNAs, including
ASH1
, were found to cofractionate with ER microsomes in a She2- and Sec3-dependent manner; thus, asymmetric mRNA transport and cortical ER inheritance are connected processes in yeast.
Publisher
American Society for Microbiology
Subject
Cell Biology,Molecular Biology
Cited by
111 articles.
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