Generation of Infectious Recombinant Human Rotaviruses from Just 11 Cloned cDNAs Encoding the Rotavirus Genome-Reference-Cited by-同舟云学术

Generation of Infectious Recombinant Human Rotaviruses from Just 11 Cloned cDNAs Encoding the Rotavirus Genome

Published:2019-04-15 Issue:8 Volume:93 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Komoto Satoshi¹^ORCID,Fukuda Saori¹,Kugita Masanori¹,Hatazawa Riona¹,Koyama Chitose²,Katayama Kazuhiko²,Murata Takayuki¹,Taniguchi Koki¹

Affiliation:

1. Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

2. Laboratory of Viral Infection I, Kitasato Institute for Life Sciences, Graduate School of Infection Control Sciences, Tokyo, Japan

Abstract

Human group A rotavirus (HuRVA) is a leading pathogen causing severe diarrhea in young children worldwide. In this paper, we describe the generation of recombinant HuRVA (strain KU) from only 11 cloned cDNAs encoding the HuRVA genome by reverse genetics. The growth properties of the recombinant HuRVA were similar to those of the parental RVA, providing a powerful tool for better understanding of HuRVA replication and pathogenesis. Furthermore, the ability to manipulate the genome of HuRVAs “to order” will be useful for next-generation vaccine production for this medically important virus and for the engineering of clinical vectors expressing any foreign genes.

Funder

Takeda Science Foundation

Mochida Memorial Foundation for Medical and Pharmaceutical Research

Japan Agency for Medical Research and Development

MEXT | Japan Society for the Promotion of Science

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02207-18

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