Author:
Unkles Shiela E.,Symington Vicki F.,Kotur Zorica,Wang Ye,Siddiqi M. Yaeesh,Kinghorn James R.,Glass Anthony D. M.
Abstract
ABSTRACT
High-affinity nitrite influx into mycelia of
Aspergillus nidulans
has been characterized by use of
13
NO
2
−
, giving average
K
m
and
V
max
values of 48 ± 8 μM and 228 ± 49 nmol mg
−1
dry weight (DW) h
−1
, respectively. Kinetic analysis of a plot that included an additional large number of low-concentration fluxes gave an excellent monophasic fit (
r
2
= 0.96), with no indication of sigmoidal kinetics. Two-dimensional (2D) and three-dimensional (3D) models of AnNitA are presented, and the possible roles of conserved asparagine residues N122 (transmembrane domain 3 ]Tm 3]), N173 (Tm 4), N214 (Tm 5), and N246 (Tm 6) are discussed.
Publisher
American Society for Microbiology
Subject
Molecular Biology,General Medicine,Microbiology
Cited by
10 articles.
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