Affiliation:
1. Groupe de Recherche sur les Antimicrobiens et Microorganismes, C.H.U. de Rouen, Hôpital Charles Nicolle, 76031 Rouen Cedex, France
Abstract
ABSTRACT
The discrimination between
Staphylococcus epidermidis
strains that contaminate and infect blood cultures is a daily challenge for clinical laboratories. The results of PCR detection of putative virulence genes were compared for contaminating strains, sepsis-related strains, catheter strains, and saprophytic strains. Multiplex PCR was used to explore the
atlE
gene, which is involved in initial adherence, the intercellular adhesion gene cluster (
ica
), which mediates the formation of the biofilm, and the
agrA
,
sarA
, and
mecA
genes, which might contribute to the pathogenicity of
S. epidermidis
. Whereas the
atlE
,
agrA
, and
sarA
genes were almost ubiquitously amplified, the
ica
and
mecA
genes were detected significantly more in infecting strains than in contaminating strains (
P
≤ 0.02) and thus appeared to be related to the potential virulence of
S. epidermidis
.
Publisher
American Society for Microbiology
Cited by
191 articles.
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