Affiliation:
1. Laboratory of Molecular Biology, National Cancer Institute, Bethesda, Maryland 20892-4255
Abstract
ABSTRACT
Lambda Xis, which is required for site-specific excision of phage lambda from the bacterial chromosome, has a much shorter functional half-life than Int, which is required for both integration and excision (R. A. Weisberg and M. E. Gottesman, p. 489–500,
in
A. D. Hershey, ed.,
The Bacteriophage Lambda
, 1971). We found that Xis is degraded in vivo by two ATP-dependent proteases, Lon and FtsH (HflB). Xis was stabilized two- to threefold more than in the wild type in a
lon
mutant and as much as sixfold more in a
lon ftsH
double mutant at the nonpermissive temperature for the
ftsH
mutation. Integration of lambda into the bacterial chromosome was delayed in the
lon ftsH
background, suggesting that accumulation of Xis in vivo interferes with integration. Overexpression of Xis in wild-type cells from a multicopy plasmid inhibited integration of lambda and promoted curing of established lysogens, confirming that accumulation of Xis interferes with the ability of Int to establish and maintain an integrated prophage.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
58 articles.
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