Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens-Reference-Cited by-同舟云学术

Critical Stages of Extracting DNA from Aspergillus fumigatus in Whole-Blood Specimens

Published:2010-10 Issue:10 Volume:48 Page:3753-3755
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

White P. Lewis¹,Perry Michael D.¹,Loeffler Juergen²,Melchers Willem³,Klingspor Lena⁴,Bretagne Stephane⁵,McCulloch Elaine⁶,Cuenca-Estrella Manuel⁷,Finnstrom Niklas⁸,Donnelly J. Peter³,Barnes Rosemary A.⁹

Affiliation:

1. Public Health Wales, Microbiology Cardiff, UHW, Heath Park, Cardiff, Wales, United Kingdom

2. Wuerzburg University, Wuerzburg, Germany

3. Radboud University, Nijmegen Medical Centre, Nijmegen, Netherlands

4. Karolinska University Hospital, Stockholm, Sweden

5. Henri Mondor Hospital, Créteil, France

6. Royal Hospital for Sick Children, Glasgow, Scotland, United Kingdom

7. National Microbiological Institute, Madrid, Spain

8. Cepheid AB, Bromma, Sweden

9. School of Medicine, Department of Infection, Immunity and Biochemistry, Cardiff University, UHW, Heath Park, Cardiff, Wales, United Kingdom

Abstract

ABSTRACT A standardized protocol for extracting DNA from Aspergillus fumigatus has been proposed by the European Aspergillus PCR Initiative (EAPCRI). Using meta-regression analysis, the EAPCRI showed certain stages of the process to be critical to providing a satisfactory analytical sensitivity. The study investigated each step of the EAPCRI protocol by elimination and monitored the influence on Aspergillus PCR performance.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01466-10

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