Affiliation:
1. Laboratoire de Bactériologie, Université de Bordeaux II, France. cbebear.@labbebear.u-bordeaux2.fr
Abstract
Fluoroquinolone-resistant mutants of Mycoplasma hominis were selected in vitro from the PG21 susceptible reference strain either by multistep selection on increasing concentrations of various fluoroquinolones or by one-step selection on agar medium with ofloxacin. The quinolone resistance-determining regions (QRDR) of the structural genes encoding the A and b subunits of DNA gyrase were amplified by PCR, and the nucleotide sequences of eight multistep-selected resistant strains were compared to those of susceptible strain PG21. Four high-level resistant mutants that were selected on norfloxacin or ofloxacin contained a C-to-T transition in the gyrA QRDR, leading to substitution of Ser-83 by Leu in the GyrA protein. Analysis of the sequence of the gyrB QRDR of the eight multistep-selected mutants did not reveal any difference compared to that of the gyrB QRDR of the reference strain M. hominis PG21. Similar analyses of eight one-step-selected mutants did not reveal any base change in the gyrA and gyrB QRDRs. These results suggest that in M. hominis, like in other bacterial species, a gyrA mutation at Ser-83 is associated with fluoroquinolone resistance.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Pharmacology (medical),Pharmacology
Reference48 articles.
1. Ahmed M. and G. E. Kenny. 1993. Decontamination of tissue cultures infected with mycoplasmas with two new fluoroquinolones: sparfloxacin and OPC 17116 abstr. G9 p. 164. In Abstracts of the 93rd General Meeting of the American Society for Microbiology 1993. American Society for Microbiology Washington D.C.
2. An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium;Bailey C. C.;J. Bacteriol.,1994
3. Characterization of genes encoding topoisomerase IV of Mycoplasma genitalium;Bailey C. C.;Gene,1996
4. Activité comparée de la minocycline et doxycycline sur les mycoplasmes pathogènes pour l'homme;Bébéar C.;Pathol. Biol.,1985
5. Neisseria gonorrhoeae acquires mutations in analogous regions of gyrA and parC in fluoroquinolone-resistant isolates;Belland R. J.;Mol. Microbiol.,1994
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