A Gene System for Glucitol Transport and Metabolism in Clostridium beijerinckii NCIMB 8052

Author:

Tangney Martin1,Brehm John K.2,Minton Nigel P.2,Mitchell Wilfrid J.1

Affiliation:

1. Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS,1 and

2. Department of Molecular Microbiology, Centre for Applied Microbiology and Research, Porton Down, Salisbury SP4 0JG,2 United Kingdom

Abstract

ABSTRACT The gutD gene of Clostridium beijerinckii NCIMB 8052 encoding glucitol 6-phosphate dehydrogenase was cloned on a 5.7-kbp chromosomal DNA fragment by complementing an Escherichia coli gutD mutant strain and selecting for growth on glucitol. Five open reading frames (ORFs) in the order gutA1 gutA2 orfX gutB gutD were identified in a 4.0-kbp region of the cloned DNA. The deduced products of four of these ORFs were homologous to components of the glucitol phosphotransferase system (PTS) and glucitol 6-phosphate dehydrogenase from E. coli , while the remaining ORF ( orfX ) encoded an enzyme which had similarities to members of a family of transaldolases. A strain in which gutD was inactivated by targeted integration lacked glucitol 6-phosphate dehydrogenase activity. The gutA1 and gutA2 genes encoded two polypeptides forming enzyme IIBC of the glucitol PTS comprising three domains in the order CBC. Domain IIA of the glucitol PTS was encoded by gutB . Glucitol phosphorylation assays in which soluble and membrane fractions of cells grown on glucose (which did not synthesize the glucitol PTS) or cells grown on glucitol were used confirmed that there is a separate, soluble, glucitol-specific PTS component, which is the product of the gutB gene. The gut genes were regulated at the level of transcription and were induced in the presence of glucitol. Cells grown in the presence of glucose and glucitol utilized glucose preferentially. Following depletion of glucose, the glucitol PTS and glucitol 6-phosphate dehydrogenase were synthesized, and glucitol was removed from the culture medium. RNA analysis showed that the gut genes were not expressed until glucose was depleted.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference35 articles.

1. Diauxic growth of Clostridium thermosaccharolyticum on glucose and xylose;Aduse-Opoku J.;FEMS Microbiol. Lett.,1988

2. Identification of two α-glucosidase activities in Clostridium acetobutylicum NCIB 8052;Albasheri K. A.;J. Appl. Bacteriol.,1995

3. Booth I. R. Mitchell W. J. (1987) Sugar transport and metabolism in the clostridia. in Sugar transport and metabolism in Gram-positive bacteria. eds ReizerJ.PeterkofskyA. (Ellis-Horwood Chichester United Kingdom) pp 165–185.

4. The pMTL nic − cloning vectors. I. Improved pUC polylinker regions to facilitate the use of sonicated DNA for nucleotide sequencing;Chambers S. P.;Gene,1989

5. Deletions generated by the transposon Tn10 in the srl recA region of the Escherichia coli chromosome;Csonka L. S.;Genetics,1979

Cited by 24 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3