Mapping and characterization of the origin of DNA replication of porcine circovirus

Author:

Mankertz A1,Persson F1,Mankertz J1,Blaess G1,Buhk H J1

Affiliation:

1. Fachbereich Gentechnik und Genetik, Robert Koch-Institut, Berlin, Germany.

Abstract

The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Reference34 articles.

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