Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes-Reference-Cited by-同舟云学术

Oxygen-regulated in vitro transcription of Rhizobium meliloti nifA and fixK genes

Published:1993-11 Issue:21 Volume:175 Page:6867-6872
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Reyrat J M¹,David M¹,Blonski C¹,Boistard P¹,Batut J¹

Affiliation:

1. Laboratoire de Biologie Moléculaire des Relations Plantes-Microorganismes, CNRS-INRA, France.

Abstract

Oxygen concentration regulates the expression of nitrogen fixation genes in the symbiotic bacterium Rhizobium meliloti. We demonstrate that two proteins, FixL and FixJ, that belong to the two-component family of regulatory proteins are necessary and sufficient for oxygen-regulated in vitro transcription of the two key regulatory genes, nifA and fixK. We show directly that FixJ is a transcriptional activator, working in conjunction with the RNA polymerase sigma 70 holoenzyme. Addition of FixL122, a soluble form of the sensor FixL protein, to the transcription assay enhanced FixJ transcriptional activity in response to low oxygen concentration. This enhancement of FixJ activity was correlated with FixJ phosphorylation.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.175.21.6867-6872.1993

Reference32 articles.

1. Oxygen regulation of nifA transcription in vitro;Agron P. G.;Proc. Natl. Acad. Sci. USA,1993

2. Prokaryotic signal transduction mediated by sensor and regulator protein pairs. Annu;Albright L. M.;Rev. Genet.,1989

3. fixK, a gene homologous with fnr and crp from Escherichia coli, regulates nitrogen fixation genes both positively and negatively in Rhizobium meliloti;Batut J.;EMBO J.,1989

4. Batut J. P. de Philip J. M. Reyrat F. Waelkens and P. Boistard. 1992. Oxygen regulation of nitrogen fixation gene expression in Rhizobium meliloti p. 183-191. In E. W. Nester and D. P. S. Verma (ed.). Advances in molecular genetics of plant-microbe interactions vol. 2. Kluver Academic Publishers Dordrecht The Netherlands.

5. In vitro activity of the nitrogen fixation regulatory protein FIXJ from Rhizobium meliloti;Batut J.;J. Bacteriol.,1991

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