Affiliation:
1. Department of Microbiology, Mount Sinai Hospital, University of Toronto, Toronto, Ontario, Canada
Abstract
ABSTRACT
To determine the validity of the rapid xylose and methyl-α-
d
-glucopyranoside (MDG) fermentation tests in distinguishing
Enterococcus gallinarum
from
Enterococcus faecium
, 156 well-characterized clinical isolates of enterococci (55
E. gallinarum
, 91
E. faecium
, and 10
Enterococcus faecalis
isolates) known to be of different clones were examined in a blinded fashion. Species identification was confirmed by PCR of the
ddl
ligase genes of
E. faecium
and
E. faecalis
and the
vanC1
gene of
E. gallinarum
. Xylose tests were performed with
d
-xylose tablets by using a heavy bacterial suspension and were interpreted after 2 h of incubation. Standard MDG fermentation tests were read after 24 h of incubation. The xylose fermentation test had a sensitivity of 98% (54 of 55) and a specificity of 99% (100 of 101) in distinguishing
E. gallinarum
from
E. faecium
and
E. faecalis
. The standard MDG test had a sensitivity of 100% (55 of 55) and a specificity of 95% (96 of 101) after 24 h. The xylose fermentation test is a simple method, easily incorporated into laboratory protocols, that distinguishes
E. gallinarum
from
E. faecium
with high sensitivity and specificity in 2 h. The standard MDG test has high sensitivity and can be useful in ruling out the presence of
E. gallinarum
but requires overnight incubation.
Publisher
American Society for Microbiology
Cited by
15 articles.
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