Author:
Bacchi C J,Lipschik G Y,Nathan H C
Abstract
Polyamines were determined by n-butanol extraction and thin-layer chromatography in four trypanosomatids: Trypanosoma brucei (rat infection) and cultures of Crithidia fasciculata, Leptomonas sp., and Trypanosoma mega. All had putrescine and spermidine but no detectable spermine. Putrescine and spermidine levels were quantitated for extracts of leptomonas during the normal growth cycle. Spermidine values peaked 18 h before peak cell populations. Spermidine-putrescine ratios for all organisms were related to the presumed phylogeny of the group.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference28 articles.
1. Polyamines during the growth in vitro ofNicotiniana glauca R. Grah. habituated tissue;Audiscio S.;Z. Pflanzenphysiol.,1976
2. Susceptibility of an insect Leptomonas and Crithidia fasciculata to several established antitrypanosomatid agents;Bacchi C. J.;Antimicrob. Agents Chemother.,1974
3. Unsubstituted agarose as an affinity matrix for isolation of NADdependent a-glycerophosphate dehydrogenase from the trypanosomatid Crithidia fasciculata;Bacchi C. J.;Biochem. Biophys. Res. Commun.,1974
4. Bachrach U. 1973. Function of naturally occurring polyamines p. 5-20. Academic Press Inc. New York.
5. Bowman I. B. R. and I. W. Flynn. 1976. Oxidative metabolism of trypanosomes p. 435-475. In W. H. R. Lumsden and D. R. Evans (ed.) Biology of the kinetoplastida vol. 1. Academic Press Inc. London and New York.
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