Carbohydrate fermentation plate medium for confirmation of Neisseria species

Author:

Faur Y C,Weisburd M H,Wilson M E

Abstract

A carbohydrate fermentation plate medium is described for rapid and reliable confirmation of Neisseria gonorrhoeae, N. meningitidis, and other Neisseria species. The medium is based on a modification of NYC (U.S. Patent 3,846,241) medium, originally designed for the isolation of pathogenic Neisseria (3). A total of 715 clinical isolates were tested for their carbohydrate fermentation reactions on the medium, in parallel with cystin-Trypticase agar medium. In 82% of the strains tested, results were in agreement on both media and 18% gave conflicting results. The NYC modification provides rapid and accurate fermentation patterns for use in routine confirmation procedures for Neisseria species.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference12 articles.

1. Modification of the rapid fermentation test for Neisseria gonorrhoeae;Brown W. J.;Appl. Microbiol.,1974

2. Carbohydrate fermentation patterns of Neisseria meningitidis determined by a microtiter method;Davies J. A.;Appl. Microbiol.,1971

3. A new medium for the isolation of pathogenic Neisseria (NYC Medium). 1. Formulation and comparisons with standard media;Faur Y. C.;Health Lab. Sci.,1973

4. A serum-free medium for testing fermentation reactions in Neisseria gonorroheae;Flynn J.;J. Clin. Pathol.,1972

5. A new fermentation medium for N. gonorrhoeae, HAP-Medium. Influence of different constituents on growth and indicator colour;Juhlin I.;Acta Pathol. Scand.,1963

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