Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay-Reference-Cited by-同舟云学术

Genotyping of Toxoplasma gondii Isolates with 15 Microsatellite Markers in a Single Multiplex PCR Assay

Published:2010-12 Issue:12 Volume:48 Page:4641-4645
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Ajzenberg Daniel¹²,Collinet Frédéric²,Mercier Aurélien²,Vignoles Philippe²,Dardé Marie-Laure¹²

Affiliation:

1. Centre National de Référence (CNR) Toxoplasmose/Toxoplasma Biological Resource Center (BRC), Centre Hospitalier-Universitaire Dupuytren, Limoges, 87042, France

2. Laboratoire de Parasitologie-Mycologie, EA 3174-NETEC, Faculté de Médecine, Université de Limoges, Limoges, 87025, France

Abstract

ABSTRACT We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers. This method was validated by testing 26 reference isolates that had been characterized with other sets of markers.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.01152-10

Reference17 articles.

1. Ajzenberg, D., A. L. Banuls, C. Su, A. Dumètre, M. Demar, B. Carme, and M. L. Dardé. 2004. Genetic diversity, clonality and sexuality in Toxoplasma gondii. Int. J. Parasitol. 34 : 1185-1196.

2. Ajzenberg, D., A. L. Banuls, M. Tibayrenc, and M. L. Dardé. 2002. Microsatellite analysis of Toxoplasma gondii shows considerable polymorphism structured into two main clonal groups. Int. J. Parasitol. 32 : 27-38.

3. Ajzenberg, D., N. Cogné, L. Paris, M. H. Bessières, P. Thulliez, D. Filisetti, H. Pelloux, P. Marty, and M. L. Dardé. 2002. Genotype of 86 Toxoplasma gondii isolates associated with human congenital toxoplasmosis, and correlation with clinical findings. J. Infect. Dis. 186 : 684-689.

4. Multiplex PCR for Typing Strains of Toxoplasma gondii

5. Ajzenberg, D., H. Yera, P. Marty, L. Paris, F. Dalle, J. Menotti, D. Aubert, J. Franck, M. H. Bessières, D. Quinio, H. Pelloux, L. Delhaes, N. Desbois, P. Thulliez, F. Robert-Gangneux, C. Kauffmann-Lacroix, S. Pujol, M. Rabodonirina, M. E. Bougnoux, B. Cuisenier, C. Duhamel, T. H. Duong, D. Filisetti, P. Flori, F. Gay-Andrieu, F. Pratlong, G. Nevez, A. Totet, B. Carme, H. Bonnabau, M. L. Dardé, and I. Villena. 2009. Genotype of 88 Toxoplasma gondii isolates associated with toxoplasmosis in immunocompromised patients and correlation with clinical findings. J. Infect. Dis. 199 : 1155-1167.

Cited by 160 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. Protozoan Diseases: Toxoplasmosis;International Encyclopedia of Public Health;2025

2. Infection dynamics following experimental challenge of pigs orally dosed with different stages of two archetypal genotypes of Toxoplasma gondii;Veterinary Parasitology;2024-08

3. Rapid genotyping of Toxoplasma gondii isolates via Nanopore-based multi-locus sequencing;AMB Express;2024-06-06

4. Detection of viable Toxoplasma gondii in retail venison;Food Control;2024-06

5. Toxoplasma gondiifrom Gabonese forest, Central Africa: first report of an African wild population;2024-05-15