Affiliation:
1. Manhattan College, New York, New York
Abstract
Beardsley, Robert E.
(Manhattan College, New York, N. Y.). Glycine resistance in
Agrobacterium tumefaciens
. J. Bacteriol.
83:
6–13. 1962.—The application of the fluctuation test of Luria and Delbrück to the distribution of glycine-resistant bacteria among cultures of
Agrobacterium tumefaciens
strain B6 indicates that resistance arises by mutation in the absence of glycine. On glycine-supplemented medium, additional resistant colonies arise during prolonged periods of incubation. Their appearance is proceded by L-form growth. In general, the number of generations over which glycine resistance is inherited in the absence of glycine is increased by serial transfers on the selection medium. In liquid medium containing glycine, sensitive bacteria form spheroplasts. Resistant bacteria continue to grow as rod forms. In the medium employed, spheroplasts are unstable.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
12 articles.
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