Complete Genome Sequence of Escherichia coli BL21-AI-Reference-Cited by-同舟云学术

Complete Genome Sequence of Escherichia coli BL21-AI

Published:2020-03-05 Issue:10 Volume:9 Page:
ISSN:2576-098X
Container-title:Microbiology Resource Announcements
language:en
Short-container-title:Microbiol Resour Announc

Author:

Bhawsinghka Niketa¹,Glenn Katie F.¹,Schaaper Roel M.¹

Affiliation:

1. Genome Integrity and Structural Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA

Abstract

Escherichia coli BL21-AI is a commercially available strain possessing a phage T7-based protein-expression system. A combination of tight regulation and high yield makes it widely used for high-level expression of toxic recombinant proteins. Here, we present the complete genome sequence of BL21-AI and provide insights on its genome.

Funder

HHS | NIH | National Institute of Environmental Health Sciences

Publisher

American Society for Microbiology

Subject

Genetics,Immunology and Microbiology (miscellaneous),Molecular Biology

Link

https://journals.asm.org/doi/pdf/10.1128/MRA.00009-20

Reference8 articles.

1. Comparative multi-omics systems analysis of Escherichia coli strains B and K-12

2. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes

3. Arabinose-induced binding of AraC protein to araI2 activates the araBAD operon promoter.