Affiliation:
1. Department of Immunology, University of Tartu, 51014 Tartu, Estonia
2. Department of Medical Microbiology, Dermatology and Infection, University of Lund, 223 62 Lund, Sweden
Abstract
ABSTRACT
Bile-tolerant
Helicobacter
species such as
Helicobacter pullorum
,
Helicobacter bilis
, and
Helicobacter hepaticus
are associated with hepatic disorders in animals and may be involved in the pathogenesis of chronic liver diseases (CLD) in humans. Antibody responses to cell surface proteins of
H. pullorum, H. bilis
, and
H. hepaticus
in serum samples from patients with CLD, a randomized population group, and healthy blood donors were evaluated by using enzyme linked immunosorbent assay (ELISA). The results were compared with the antibody responses to
Helicobacter pylori
. For analysis of a possible cross-reactivity between bile-tolerant
Helicobacter
species and
H. pylori
, sera from a subpopulation of each group were absorbed with a whole-cell extract of
H. pylori
and retested by ELISA. Results before absorption showed that the mean value of the ELISA units for
H. pullorum
was significantly higher in patients with CLD than in healthy blood donors (
P
= 0.01). Antibody reactivity to cell surface protein of
H. hepaticus
was also significantly higher in the CLD patients than in the healthy blood donors and the population group (
P
= 0.005 and
P
= 0.002, respectively). Following the absorption, antibody responses to
H. pullorum
decreased significantly in all three groups (
P
= 0.0001 for CLD patients,
P
= 0.0005 for the population group, and
P
< 0.0001 for the blood donors), indicating that cross-reactivity between
H. pylori
and other
Helicobacter
spp. occurs. The antibody responses to
H. hepaticus
and
H. bilis
in CLD patients remained high following absorption experiments compared to ELISA results before absorption. The significance of this finding requires further investigations.
Publisher
American Society for Microbiology
Subject
Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy
Cited by
34 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献