Biosensor Detection of Botulinum Toxoid A and Staphylococcal Enterotoxin B in Food

Author:

Sapsford Kim E.1,Taitt Chris R.2,Loo Nicole2,Ligler Frances S.2

Affiliation:

1. George Mason University, 10910 University Blvd., MS 4E3, Manassas, Virginia 20110

2. Center for Bio/Molecular Science & Engineering, Naval Research Laboratory, Washington, DC 20375-5438

Abstract

ABSTRACT Immunoassays were developed for the simultaneous detection of staphylococcal enterotoxin B and botulinum toxoid A in buffer, with limits of detection of 0.1 ng/ml and 20 ng/ml, respectively. The toxins were also spiked and measured in a variety of food samples, including canned tomatoes, sweet corn, green beans, mushrooms, and tuna.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference31 articles.

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5. Choi, K., W. Seo, S. Cha, and J. Choi. 1998. Evaluation of two types of biosensors for immunoassay of botulinum toxin. J. Biochem. Mol. Biol.31:101-105.

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