Immunolabeling of lipopolysaccharide liberated from antibiotic-treated Escherichia coli

Author:

Flynn P M1,Shenep J L1,Gigliotti F1,Davis D S1,Hildner W K1

Affiliation:

1. Department of Infectious Diseases, St. Jude Children's Research Hospital, Memphis, Tennessee 38101.

Abstract

Increased anti-core glycolipid antibody binding was visualized by immunoelectron microscopy after incubation of Escherichia coli K1:O7 cells with a bacteriolytic antibiotic and compared with binding in control cells. The findings suggest that the core glycolipid regions of the lipopolysaccharides of some gram-negative bacilli can be effectively sequestered until liberated by antibiotic-induced cell lysis.

Publisher

American Society for Microbiology

Subject

Infectious Diseases,Immunology,Microbiology,Parasitology

Reference13 articles.

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2. Development of murine monoclonal antibodies to Pneumocystis carinii;Gigliotti F.;J. Infect. Dis.,1986

3. Endotoxemia induced by antibiotic therapy: a mechanism for adrenal corticosteroid protection in gram-negative sepsis;Johnston C. A.;Trans. Assoc. Am. Phys.,1984

4. Localization of viral nucleocapsids using immunoelectronmicroscopy;Murti K. G.;J. Electron Microsc. Tech.,1985

5. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Site of synthesis of lipopolysaccharide;Osborn M. J.;J. Biol. Chem.,1972

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