Neomycin resistance as a selectable marker in Methanococcus maripaludis-Reference-Cited by-同舟云学术

Neomycin resistance as a selectable marker in Methanococcus maripaludis

Published:1996-11 Issue:11 Volume:62 Page:4233-4237
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Argyle J L¹,Tumbula D L¹,Leigh J A¹

Affiliation:

1. Department of Microbiology, University of Washington, Seattle 98195-7242, USA.

Abstract

We cloned the aminoglycoside phosphotransferase genes APH3'I and APH3'II between the Methanococcus voltae methyl reductase promoter and terminator in a plasmid containing a fragment of Methanococcus maripaludis chromosomal DNA. The resulting plasmids encoding neomycin resistance transformed M. maripaludis at frequencies similar to those observed for pKAS102 encoding puromycin resistance. The antibiotic geneticin was not inhibitory to M. maripaludis.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/aem.62.11.4233-4237.1996

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