Systematic Evaluation of the Viable Microbiome in the Human Oral and Gut Samples with Spike-in Gram+/

Systematic Evaluation of the Viable Microbiome in the Human Oral and Gut Samples with Spike-in Gram+/– Bacteria

Published:2023-04-27 Issue:2 Volume:8 Page:
ISSN:2379-5077
Container-title:mSystems
language:en
Short-container-title:mSystems

Author:

Liu Feng¹,Lu Hui¹²,Dong Biao¹,Huang Xiaochang¹,Cheng Hongyu¹,Qu Ru¹,Hu Yichen¹,Zhong Luyun³,Guo Zhenni⁴,You Yuehua⁵⁶,Xu Zhenjiang Zech¹^ORCID

Affiliation:

1. State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang, People’s Republic of China

2. Jiangxi Agricultural University, College of Bioscience and Bioengineering, Nanchang, People’s Republic of China

3. Department of Stomatology, Second Affiliated Hospital of Nanchang University, Nanchang, People’s Republic of China

4. Shenzhen Wanhe Pharmaceutical Co., Ltd., Shenzhen, People’s Republic of China

5. Department of Stomatology, Longhua People’s Hospital Affiliated to Southern Medical University, Shenzhen, People’s Republic of China

6. School of Stomatology, Southern Medical University, Guangzhou, People’s Republic of China

Abstract

The functions and phenotypes of microbial communities are largely defined by viable microbes. Through advanced nucleic acid sequencing technologies and downstream bioinformatic analyses, we gained an insight into the high-resolution microbial community composition of human saliva and feces, yet we know very little about whether such community DNA sequences represent viable microbes.

Funder

Foundation for Innovative Research Groups of the National Natural Science Foundation of China

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/msystems.00738-22

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