One-Cell Metabolic Phenotyping and Sequencing of Soil Microbiome by Raman-Activated Gravity-Driven Encapsulation (RAGE)

Author:

Jing Xiaoyan123,Gong Yanhai123,Xu Teng123,Meng Yu123,Han Xiao123,Su Xiaolu123,Wang Jianmei123,Ji Yuetong143,Li Yuandong123,Jia Zhongjun53ORCID,Ma Bo123,Xu Jian123ORCID

Affiliation:

1. Single-Cell Center, CAS Key Laboratory of Biofuels, Shandong Key Laboratory of Energy Genetics and Shandong Institute of Energy Research, Qingdao Institute of BioEnergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong, China

2. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, Shandong, China

3. University of Chinese Academy of Sciences, Beijing, China

4. Qingdao Single-cell Biotechnology Co., Ltd., Qingdao, Shandong, China

5. State Key Laboratory of Soil and Sustainable Agriculture, Institute of Soil Science, Chinese Academy of Sciences, Nanjing, Jiangsu, China

Abstract

Soil is home to an enormous and complex microbiome that features arguably the highest genomic diversity and metabolic heterogeneity of cells on Earth. Their in situ metabolic activities drive many natural processes of pivotal ecological significance or underlie industrial production of numerous valuable bioactivities.

Publisher

American Society for Microbiology

Subject

Computer Science Applications,Genetics,Molecular Biology,Modeling and Simulation,Ecology, Evolution, Behavior and Systematics,Biochemistry,Physiology,Microbiology

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